Test No. 444A: In Vitro Immunotoxicity

IL-2 Luc Assay

This Test Guideline (TG) describes the IL-2 Luc Assay test method to evaluate the potential immunotoxic effects of chemicals on T lymphoblastic cell line. This cell line allows quantitative measurement of luciferase gene induction by detecting luminescence from well-established light producing luciferase substrates as indicators of the activity of IL-2, IFN-γ and GAPDH in cells following exposure to immunotoxic chemicals. The method is intended to be used as a part of a battery to determine immunotoxic potential of chemicals.

04 Jul 2023 28 pages English Also available in: French

https://doi.org/10.1787/27b10ba3-en 9789264934740 (PDF)

Author(s): OECD